Skip to main content

Tag: genetics

TIssue Culture

Why Tissue Culture Matters Now for the Future of Controlled Environment Agriculture

Tissue culture is becoming a practical tool for growers who want cleaner starting material, more consistent genetics, and a pathway into higher value crops. As the industry matures, knowing where tissue culture fits into production planning is becoming increasingly relevant.

I recently met with the group leading our first dedicated tissue culture session at Indoor Ag-Con. Hearing their combined experience created a clearer picture of how this work already supports growers who want predictable and healthy plants. This panel brings together hands-on operators who deal with real production conditions every day.

The session will be moderated by Della Fetzer, founder and CEO of Rebel Cultures. Her work spans conservation, agriculture, forestry, laboratory design, and tissue culture project execution. Joining her are:

  • Rinnie Rodenius, Co-Owner and Head of Operations, Polymorph Bio. Her background includes commercial work across house plants, landscape plants, and endangered orchids, with experience managing clean stock programs and solving contamination challenges across multiple crop types.
    Micah E. Stevens, Ph.D., Research Lab Manager, Sierra Gold Nurseries. His work focuses on genetic testing, woody plant micropropagation, and protocol development to support their commercial tissue culture program.
    Dr. Hsien Ming Easlon, micropropagation specialist with extensive commercial experience across multiple high value crops.

Together they have worked across more than 1,400 plant varieties and have built or managed tissue culture programs that support growers at scale.

With that group in mind, here is how tissue culture fits into the broader CEA conversation.

Tissue culture gives growers a more dependable start

A consistent theme across the panel was how important it is to begin with clean, uniform plants. Many higher value crops grown indoors depend on vegetative starts rather than seed, which makes the condition of the starting material a major factor in overall success. Vegetative starts can include cuttings, runners, rhizomes, bare roots, tissue culture, and other methods that require clean and consistent plant material from the beginning.

Tissue culture helps growers access clean stock programs, steady supplies of starts, and plant material that behaves more predictably in controlled environments. These advantages support crop scheduling, planning, and consistent yields.

Rinnie Rodenius explained that tissue culture has been used for decades to solve problems growers still face today. Issues like virus load, decline in mother plants, uneven vegetative material, and slow rollout of new genetics all trace back to reliability at the start. Tissue culture helps stabilize these areas and gives growers a stronger foundation.

Higher value crops require a different level of cleanliness

Blackberry Tissue CultureAs growers move into crops such as strawberries, cane berries, wasabi, and specialty ornamentals, many discover that traditional propagation brings limitations. Pathogens spread easily, mother plants break down over time, and plants can behave inconsistently when the starting material is not clean.

Dr. Hsien Ming Easlon’s work across strawberry, raspberry, blueberry, coffee, agave, and other crops shows how important clean stock is when scaling production. Indoor systems depend on plants responding predictably to the environment around them. Tissue culture supports that predictability by providing uniform, disease-free plants at volume.

Tissue culture creates opportunities for new crop categories

Rebel Cultures highlighted an important point. Some crops mature too slowly from seed or do not perform well when started the traditional way. Tissue culture can shorten timelines and create a clean starting point for plants that often struggle indoors.

This opens the door for growers interested in diversifying into higher value crops. Berries, wasabi, squash, and other specialty categories become more realistic when the starting material is clean, uniform, and ready for controlled production.

A wider range of crops also strengthens the industry as a whole. More options give growers flexibility and reduce dependence on only a few crop types.

Growers need clear guidance on how and when to use tissue culture

Every panelist pointed out the amount of confusion surrounding tissue culture. There is genuine interest, but many growers are unsure how to start, what timelines look like, or how to evaluate a potential partner.

Micah Stevens emphasized the importance of correct testing, proper scaling, and avoiding common mistakes that slow early projects down. This is where experienced practitioners become valuable. They help growers plan correctly, understand realistic timelines, and determine whether tissue culture is a good fit for their crop and business model.

The group leading this session offers guidance shaped by real-world production. They each operate or have operated functioning labs that supply commercial growers, and their perspectives come from solving practical challenges rather than theory.

What this means for CEA operators

Growers do not need to run a tissue culture lab to benefit from one. What matters is knowing:

  • How clean starting material affects production
    • When tissue culture supports a crop choice
    • How to choose a credible lab partner
    • What questions to ask before beginning a project
    • How tissue culture fits within existing propagation systems

As operators explore higher value crops, these questions naturally become part of the planning process. Tissue culture gives growers a path to stable genetics, cleaner supply chains, and a more dependable foundation for intensive indoor production.

Indoor Ag-Con is committed to bringing these conversations to the industry in a clear and practical way. This session is designed to give growers guidance they can use immediately as they evaluate their next steps.

 Special thanks to Della Fetzer, Rebel Cultures and  Dr. Hsien Ming Easlon for photos.

 

Tracy Lee Sakata Seed America

Bridging The Gap Between Genetics and Indoor Growing Needs: Sakata Seed Cultivates CEA Success

Q&A With Tracy Lee Zogby, Head of the Controlled Environment Agriculture Department, Sakata Seed America

Sakata Seed Indoor Ag-ConHarnessing the power of existing genetics and strategic collaborations, Sakata Seed is providing quality seeds, exceptional service, and robust support to meet the diverse needs and demands of the rapidly expanding controlled environment agriculture (CEA) market.  We had the chance to catch up with Tracy Lee Zogby, CEA Lead, Sakata Seed America, to learn more about the company’s goals and objectives for its CEA department. We talk about some of Sakata’s outstanding varieties, the challenges and opportunities that CEA presents, upcoming advancements and innovations in seed development for indoor growers and more.

Sakata SeedTell us about the main goals and objectives of Sakata Seed’s CEA division?  How does it support the needs and demands of greenhouse grower, vertical farm operators  and other indoor growers in the sector?

In 2019, Sakata Seed America began to pivot focus to an array of existing genetics that show exceptional promise in the CEA market.  We quickly noticed the synergy between the emerging and exponentially growing CEA market and our existing crop business groups. Currently, we are partnering with key market growers to extensively trial existing worldwide Sakata genetics that showed promise for indoor and CEA environments. Through strategic collaborations, exceptional genetics, and continued breeding efforts, coupled with expansion and investment in our Research & Development team, we feel well-equipped to grow our CEA partnerships and supply quality seed in strong supply to the category, backed by excellent service and support.

Last year, to further support the needs of the indoor grower, we launched SakataCEA.com – a full-scale, online resource cataloging of all that Sakata’s CEA program has to offer. It features the entire CEA product portfolio, downloadable literature materials and tools for cultivation success, a digital sample ordering form and trialing information, a fully responsive seed distributor map, and a staff directory for easy access to product support.

 

Could you provide a couple of examples of Sakata Seed’s varieties that have shown exceptional performance in indoor growing environments, and what attributes make them well-suited for CEA?

Growers have found success with several of our varieties across varied technologies. A few standouts include Mizuna Mustard Miz America, Kale Blue Ridge, and Pak Choi Joi Choi. Miz America, for example, displays an unparalleled intense color and mild flavor that makes it an ideal component in salad blends. Kale Blue Ridge has shown exceptional yields and uniformity as well as a desirable leaf texture and color. Pak Choi Joi Choi, when grown to a baby leaf stage, is flavorful, attractive, and high yielding, in addition to showing notably high post-harvest quality.

 

Sakata SeedShare some insights into the unique challenges and opportunities that CEA presents for seed development and production, compared to traditional outdoor farming?

Although grouped under the single broad category of ‘CEA’, indoor growers are extremely diverse in their crop needs, growing conditions, facility types and technology. I would say one of the biggest challenges in seed development is deciding exactly which traits to breed for. Success with one type of grower or technology does not translate into success with all CEA growers, even within the same facility type. We must be able to develop genetics that fulfill the broader common traits needed across the largest number of growers and then depend on growers to manipulate their different technologies and growing methods to control more individual needs within this broader framework.

However, one of the most potentially exciting opportunities that I see in developing future varieties is the ability to focus more of our breeding on consumer-driven traits such as flavor or nutrition over chasing some of the disease resistance needs for outdoor farming.

For seed production, the biggest challenge is clarity around the entire process.  Producing seed is a time intensive process, anywhere from 1 to 2 years from stock seed increases through to cleaning and testing the final crop. Having transparency in understanding the CEA growers’ seed needs in advance, both in timing and quantities, is crucial to planning and providing consistent supplies.

 

Sakata SeedHow do you identify and prioritize the traits and characteristics that are more important for indoor growers — are you able to collaborate with  growers  to gather feedback and insights?

At times it can be challenging to collect meaningful outside trial data to track our progress toward goals. I would like to see increased direct input from growers and eventually see some uniformity in growing systems and clearer overarching breeding goals.  We have seen the greatest successes and fastest progress when we are able to develop a trusting, open, mutually beneficial trialing collaboration with growers.

 

Sakata SeedCan you highlight any recent advancements or innovations in seed development within your CEA division?  What varieties or technologies can growers anticipate from Sakata Seed in the near future.

Lettuce, arugula, beet greens, mustard, and pack choi are a few of the crops on which we are gathering data and breeding,  both allowing for crossover commercial products to be used now and for key trait identification for next-generation breeding projects in the pipeline. Our breeders are analyzing traits that will allow for optimum yield, advantageous plant architecture, and noteworthy flavor and texture while displaying a spectrum of colors and textures for consumer interest.